Baetisca laurentina McDunnough is reported for the first time from West Virginia. One male imago was collected near Twelvepole Creek, Wayne County, West Virginia. This record extends the range of this species eastward to the Mid-Atlantic coastal region. New distributional records (57) of Baetisca spp. are reported for Kentucky (15) and West Virginia (42). The following county records have been added to the list of Baetisca spp. from the two states: Baetisca berneri Tarter and Kirchner (KY/1 and WV/13), B. carolina Traver (WV/3), B. gibbera (WV/1), B. lacustris McDunnough KY/14 and WV/22), B. laurentina McDunnough (WV/1), and B. rubescens Provancher (WV/2). Baetisca lacustris is the most widespread baetiscid in West Virginia (26 counties) and Kentucky (25 counties). This species was found in six drainage basins (I, II, IV, V, VI, VII) in West Virginia. Only one baetiscid mayfly, B. rubescens, was recorded for drainage basin III.